Screening, Identification, and Optimization of Enzyme-Producing Conditions for Cellulose-Degrading Bacteria in Distillery Lees

Cellulose-degrading bacteria were identified from distillery lees, and the strains optimized for fermentation enzyme production, providing effective resource utilization of lees developing cellulase. Based on univariate test, response surface test was used to optimize production conditions fermentation. The screened strain JZ2 had a clear circle-to-colony diameter ratio 2.0. activities exoglucanase, endoglucanase, β-glucosidase 4.341 ± 0.05 U/mL, 1.874 0.04 0.739 0.02 respectively. bacterial colonies large, cells’ morphology rod-shaped. We Bacillus cereus (JZ2) 16S rDNA sequence homology phylogenetic tree analysis as belonging genus in thick-walled phylum. optimum cellulase time 2 d, rotation rate 180× g, temperature 26 °C using method. activity 4.625 U/mL under optimal conditions. In addition good activity, may have potential convert cellulose into useful compounds.